文獻(xiàn)：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文獻(xiàn)鏈接：https://onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

Our previous study has revealed that TFPI-2 is expressed at a low level in NPC cells and tissues [16]. As proof of concept we use FA as targeting molecules, which conjugated to amino-terminated polyethylene glycol (NH2-PEG-COOH) and polyethylenimine (PEI) to perform cationic polymers (FA-PEG-PEI) via amidation. Aldehyde sodium alginate modified Fe3O4 magnetic particles coated cisplatin (SPION-CDDP) was constructed with the above cationic polymer to obtain composite carriers (FA-PEG-PEI@SPION-CDDP), which then adsorbed TFPI-2 pDNA via electrostatic interaction to obtain the FA-targeted, CDDP, and TFPI-2-coated nanocomposites (FA-PEG-PEI@SPION-CDDP-TFPI-2). In vitro assays indicated that the novel compounds possessed excellent load-carrying capacity and drug stability and showed negligible cytotoxicity and enhanced targetability to FR positive (FR+) NPC HNE-1 cells.

研究表明，TFPI-2在鼻咽癌細(xì)胞和組織中的表達(dá)水平較低。作為概念驗(yàn)證，我們使用FA作為靶向分子，它與氨基封端的聚乙二醇（NH2-PEG-COOH）和聚乙烯亞胺（PEI）共軛，通過酰胺化形成陽離子聚合物（FA-PEG-PEI）。

用上述陽離子聚合物構(gòu)建了醛-海藻酸鈉改性的Fe3O4磁性粒子包覆順鉑（SPION-CDDP），以獲得復(fù)合載體(FA-PEG-PEI@SPION-CDDP)，然后通過靜電相互作用吸附TFPI-2 pDNA，獲得FA靶向、CDDP和TFPI-2涂覆的納米復(fù)合材料(FA-PEG-PEI@SPION-CDDP-TFPI-2).體外試驗(yàn)表明，新化合物具有優(yōu)異的承載能力和藥物穩(wěn)定性，對FR陽性（FR+）NPC HNE-1細(xì)胞的細(xì)胞毒性可以忽略不計(jì)，靶向性增強(qiáng)。

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