文獻：Development of PLGA-PEG-COOH and Gelatin-Based Microparticles Dual Delivery System and E-Beam Sterilization Effects for Controlled Release of BMP-2 and IGF-1

作者：Yan Bai, Seyedsina Moeinzadeh, Sungwoo Kim, Youngbum Park, Elaine Lui, Hua Tan, Weiling Zhao, Xiaobo Zhou, Yunzhi Peter Yang

文獻鏈接：https://onlinelibrary.wiley.com/doi/abs/10.1002/ppsc.202000180

摘要：

A poly(lactic-co-glycolic acid)-poly(ethylene glycol)-carboxyl (PLGA-PEG-COOH) and gelatin-based microparticles (MPs) dual delivery system for release of bone morphogenetic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) is developed. The delivery system is characterized based on its morphology, loading capacity, encapsulation efficiency, and release kinetics. The effects of electron beam (EB) sterilization on BMP-2 and IGF-1-loaded MPs and their biological effects are examined. The synergistic effect of a controlled dual release of BMP-2 and IGF-1 on osteogenesis of human mesenchymal stem cells (MSCs) is evaluated. Encapsulation efficiency of growth factors into gelatin and PLGA-PEG-COOH MPs is in the range of 64.78% to 76.11%. E-beam sterilized growth factor delivery systems are effective in significantly promoting osteogenesis of MSCs, although E-beam sterilization decreases the bioactivity of growth factors in MPs by ≈22%. BMP-2 release behavior from gelatin MPs/PEG hydrogel shows a faster release (52.7%) than that of IGF-1 from the PLGA-PEG-COOH MPs/PEG hydrogel (27.3%). The results demonstrate that the gelatin and PLGA-PEG-COOH MPs-based delivery system can realize temporal release of therapeutic biomolecules by incorporating different growth factors into distinct microparticles. EB sterilization is an accessible method for sterilizing growth factors-loaded carriers, which can pave the way for implementing growth factor delivery in clinical applications.

開發了一種用于釋放骨形態發生蛋白-2（BMP-2）和胰島素樣生長因子-1（IGF-1）的聚乳酸-聚乙二醇-羧基（PLGA-PEG-COOH）和明膠基微粒（MP）雙遞送系統。

遞送系統的特征在于其形態、負載能力、包封效率和釋放動力學。研究了電子束（EB）滅菌對負載BMP-2和IGF-1的MP的影響及其生物學效應。評估了BMP-2和IGF-1的受控雙重釋放對人間充質干細胞（MSCs）成骨的協同作用。

生長因子在明膠和PLGA-PEG-COOH MP中的包封效率在64.78%至76.11%之間。電子束滅菌的生長因子遞送系統能有效顯著促進MSCs的成骨，盡管電子束滅菌會使MP中生長因子的生物活性降低約22%。

明膠MPs/PEG水凝膠的BMP-2釋放行為比PLGA-PEG-COOH MPs/PEG凝膠的IGF-1釋放行為（27.3%）更快（52.7%）。結果表明，基于明膠和PLGA-PEG-COOH MP的遞送系統可以通過將不同的生長因子摻入不同的微粒中來實現治療性生物分子的暫時釋放。

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