標題功能型聚乙二醇化殼聚糖納米顆粒的制備和表征

作者肖巨穎; 趙霞; 林園; 蘇朝暉

文獻鏈接：https://openurl.ebsco.com/EPDB%3Agcd%3A14%3A17421374/detailv2?sid=ebsco%3Aplink%3Acrawler&id=ebsco%3Adoi%3A10.19894%2Fj.issn.1000-0518.240425&link_origin=none

摘要：

In the present study, Carboxyl and azide terminated polyethylene (N3-PEG-COOH) with different relative molecular mass of 500 and 1000 were grafted onto the chitosan (CS) backbones through amide reaction, followed by the preparation of chitosan-polyethylene glycol (CS-PEG) nanoparticles with controllable size and zeta potential via sodium tripolyphosphate (TPP) ionotropic gelation. Characterized by Fourier transform infrared spectrometer (FT-IR), nuclear magnetic resonance spectroscopy (NMR) and thermal gravimetric analyzer (TGA) confirmed the successful incorporation of PEG, and the structures of the nanoparticles were analyzed by transmission electron microscopy (TEM) and nanoparticle size and zeta potential analyzer. The results demonstrated that when m (CS) ∶m (TPP) is 3.33∶1, CS nanoparticles show excellent structural stability. Based on this, increasing the PEG grafting density further reduces the particle size and surface potential of CS-PEG nanoparticles. Moreover, the chemical reactivity of azide in CS-PEG nanoparticles was proved by the click reaction with alkyne-modified fluorescent molecule (5-FAM-alkyne), showing their potential as platform for target drug delivery, fluorescent probes and other biomedical applications.

在本研究中，通過酰胺反應將相對分子質量為500和1000的羧基和疊氮封端的聚乙烯（N3-PEG-COOH）接枝到殼聚糖（CS）骨架上，然后通過三聚磷酸鈉（TPP）離子凝膠化制備尺寸和ζ電位可控的殼聚糖-聚乙二醇（CS-PEG）納米粒子。通過傅里葉變換紅外光譜儀（FT-IR）、核磁共振光譜（NMR）和熱重分析儀（TGA）的表征證實了PEG的成功摻入，并通過透射電子顯微鏡（TEM）和納米粒子尺寸和ζ電位分析儀分析了納米粒子的結構。

結果表明，當m（CS）∶m（TPP）為3.33∶1時，CS納米粒子表現出優異的結構穩定性。基于此，增加PEG接枝密度進一步降低了CS-PEG納米顆粒的粒徑和表面電位。此外，通過與炔烴修飾的熒光分子（5-FAM-炔烴）的點擊反應，證明了疊氮化物在CS-PEG納米顆粒中的化學反應性，顯示了它們作為靶向藥物遞送、熒光探針和其他生物醫學應用平臺的潛力。

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