文獻：紅細胞內化精氨酸-甘氨酸-天冬氨酸(iRGD)修飾增強胃癌放射*療效

鏈接：https://pmc.ncbi.nlm.nih.gov/articles/PMC9660045/

作者：周沖 ,劉勤 ,孟凡彥 ,丁乃清 ,景艷 ,劉寶瑞

節選：

采用1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-聚乙二醇-iRGD（DSPE-PEG-iRGD）通過脂質插入法修飾紅細胞（RBC），無需直接化學生物偶聯。通過共聚焦顯微鏡觀察熒光染料示蹤功能性紅細胞。體外穩定性評估：將紅細胞置于細胞培養基中孵育48小時，然后進行熒光衰減試驗。此外，構建了MKN-45細胞皮下移植癌細胞小鼠模型，以評估DSPE-PEG-iRGD修飾紅細胞（RBC-iRGD）的靶向藥效和靶向增強效應。

1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-poly ethylene glycol-iRGD (DSPE-PEG-iRGD) was used to modify RBCs by a lipid-insertion method without direct chemical bioconjugation. Fluorescent dyes were used to trace the functional RBCs through confocal microscopy examination. In vitro stability evaluation was performed using cell culture medium incubation for 48 h followed by fluorescence decay assay. Furthermore, a subcutaneous cancer cell mouse model was constructed with MKN-45 cells for target efficacy and RT enhancement evaluation with DSPE-PEG-iRGD-modified RBCs (RBC-iRGD).

西安齊岳生物提供相關產品：

DSPE-PEG-Estrogen

DMPE-PEG-Silane

DSPE-PEG-Pyrene

DSPE-PEG-Silane

DSPE-PEG-Dextran

m-PEG8-DSPE

DSPE-PEG-His

Peptides PEG PCL

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