文獻:Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes
作者:Nao Hiramoto-Yamaki, Kenji A. K. Tanaka, Kenichi G. N. Suzuki, Koichiro M. Hirosawa, Manami S. H. Miyahara, Ziya Kalay, Koichiro Tanaka, Rinshi S. Kasai … See all authors
文獻鏈接:
https://onlinelibrary.wiley.com/doi/full/10.1111/tra.12163Effects on DeffMACRO of Cy3-DOPE in the PtK2 PM
The DeffMACRO values of Cy3-DOPE were significantly increased and decreased by 30%, after the cells were treated with 5?μm latrunculin-B and 25?μm jasplakinolide, respectively (Figure 4, bottom right; Table 1), consistent with our previous observations in other cell types 19, 45, 48. These drug concentrations were much higher than those used previously, but PtK2 cells were extremely resistant to these drugs, and did not exhibit any morphological changes even under these conditions. Considering these results, along with the result showing a 20-fold increase of the Cy3-DOPE DeffMACRO value upon blebbing?+?actin depletion (Table 1), we concluded that the actin filaments associated with the PM cytoplasmic surface are responsible for slowing the diffusion of Cy3-DOPE in the PtK2 PM, as found previously in other cell types
PtK2粉末冶金中Cy3-DOPE對DeffMACRO的影響
在用5μmol/L的濃度處理細胞后,Cy3-DOPE的DeffMACRO值顯著增加和減少了30%?μm latrunculin-B和25?μm jasplakinolide(圖4,右下;表1),與我們之前在其他細胞類型19、45、48中的觀察結果一致。這些藥物濃度遠高于以前使用的藥物濃度,但PtK2細胞對這些藥物具有極強的耐藥性,即使在這些條件下也沒有表現出任何形態變化。
考慮到這些結果,以及起泡后Cy3 DOPE DeffMACRO值增加20倍的結果?+?肌動蛋白耗竭,我們得出結論,與PM細胞質表面相關的肌動蛋白絲負責減緩PtK2 PM中Cy3-DOPE的擴散,正如之前在其他細胞類型中發現的那樣。
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