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近紅外熒光染料IRDye 650 NHS Ester

IR 650 NHS酯（IRDye® 650 NHS酯類似物）是一種水溶性，明亮，遠紅熒光染料，光譜上類似于用于流式細胞術，顯微鏡和其他應用的其他650染料。IR 650 共軛物非常適合 633 nm 或 647 nm 激光線。與其他熒光團相比，使用長波長染料（如Cy5®染料或Alexa Fluor® 647染料）的顯著優勢是生物標本在該光譜區域中的自發熒光較低。

NHS酯（或琥珀酰亞胺酯）是最常用的胺反應基團，用于標記蛋白質伯胺（Lys），胺修飾寡核苷酸和其他含胺分子。蛋白質可以用IR 650 NHS酯以高摩爾比標記，而不會顯著的自熄滅，從而導致更亮的偶聯物和更靈敏的檢測。IR 650偶聯物可用于檢測低豐度靶標。

　　ReactivityPrimary amine

　　Abs/Em Maxima651/668 nm

　　Extinction coefficient230,000 cm-1M-1

　　SolubilityWater, DMSO, DMF

　　Spectrally similar dyesCy5, Alexa Fluor® 647, DyLight® 649,

　　Molecular weight1032.18

　　Storage conditions-20C

　　Shipping conditionsAmbient temperature

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