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IRDye 680RD Carboxylic acid

　　IRDye 680RD infrared dye is the near-infrared fluorescent dye of choice for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals.

　　This infrared dye is ideal for labeling proteins, peptides, and antibodies. IRDye 680RD dye conjugates are optimized for protein detection applications, including quantitative Westerns and In-Cell Western™ assays. When used with IRDye 800CW dye-labeled agents, IRDye 680RD dye conjugates enable two-color imaging.

　　In-Cell Western Assay normalization with IRDye 680RD secondary antibody. A431 cells were treated with 2-fold serial dilutions of EGF (800-1.6 ng/mL). After fixation and permeabilization, cells were probed with rabbit anti-phospho EGFR and Mouse anti-ERK2 followed by detection with IRDye 800CW Goat anti-Rabbit IgG (P/N 926-32211) and IRDye 680RD Goat anti-Mouse IgG (P/N 926-68070). Column 1: background (no primary antibody); Columns 2-11: 2-fold dilution EGF treatment; Column 12: ** standard (no EGF treatment).