文獻(xiàn)：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文獻(xiàn)鏈接：https://onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

The FA-PEG-PEI@SPION-CDDP was constructed with SPION-CDDP (core) and FA-PEG-PEI (shell). Numerous detached electropositive amino groups in the shell covered SPION-CDDP nanoparticles were able to electrostatically adsorb electronegative TFPI-2 pDNA. To explore the binding ability of FA-PEG-PEI@SPION-CDDP with TFPI-2 pDNA, gel electrophoresis was observed in Figure 3(a). It was found that with the increasing mass ratio of FA-PEG-PEI@SPION-CDDP/TFPI-2 {Figure 3(a)~B (1?:?4) → C (1?:?2) → D (1?:?1)}, more TFPI-2 was blocked in sample well. When the mass ratio was equal to or more than 2 (Figure 3(a)~E), TFPI-2 pDNA was restricted completely in the well, indicating entirely adsorption and encapsulation by FA-PEG-PEI@SPION-CDDP. As a result, the relative mass ratio was determined as 2?:?1 to synthesize the final complex. To investigate the protection of TFPI-2-loaded complex (FA-PEG-PEI@SPION-CDDP-TFPI-2) against DNase-I degrading, FA-PEG-PEI@SPION-CDDP was mixed with overdose TFPI-2, then various content of DNase-I was added to run electrophoresis. As shown in Figure 3(b), compared with TFPI-2 migration without DNase-I in Figure 3(a)~B, the more DNase-I was added, the more detached TFPI-2 was degraded with more fade band of TFPI-2 migration. When DNase-I was equal to or higher than 15 units, the detached TFPI-2 (Figure 3(b)~J, K) was entirely digested except for the sample well. This evidence shed light on the enzymatic hydrolysis of DNase-I in TFPI-2 pDNA and indicated that FA-PEG-PEI@SPION-CDDP is feasible in adsorbing TFPI-2 and protecting pDNA from degradation. Thus pDNA could avoid digestion by DNase in media or blood before reaching the targeted cells, leading to an efficient transfection in tumors.

這個(gè)FA-PEG-PEI@SPION-CDDP用SPION-CDDP（核心）和FA-PEG-PEI（外殼）構(gòu)建。殼覆蓋的SPION-CDDP納米顆粒中的許多分離的正電氨基能夠靜電吸附電負(fù)性TFPI-2 pDNA。探索其結(jié)合能力FA-PEG-PEI@SPION-CDDP對于TFPI-2 pDNA，凝膠電泳。

發(fā)現(xiàn)隨著質(zhì)量比的增加FA-PEG-PEI@SPION-CDDP/TFPI-2{圖3（a）~B（1:4）→C（1:2）→D（1:1）}，更多的TFPI-2在樣品孔中被阻斷。當(dāng)質(zhì)量比等于或大于2時(shí)，TFPI-2 pDNA完全限制在孔中，表明完全被吸附和包封FA-PEG-PEI@SPION-CDDP.結(jié)果，相對質(zhì)量比被確定為2?:?1 合成最終的復(fù)合物。

TFPI-2負(fù)載復(fù)合物的保護(hù)作用研究(FA-PEG-PEI@SPION-CDDP-TFPI-2)抗DNase-I降解，F(xiàn)A-PEG-PEI@SPION-CDDP與過量TFPI-2混合，然后加入不同含量的DNase-I進(jìn)行電泳。

中沒有DNase-I的TFPI-2遷移相比，添加的DNase-I越多，TFPI-2的降解程度越高，TFPI-1遷移的衰減帶越多。當(dāng)DNase-I等于或高于15個(gè)單位時(shí)，除樣品孔外，分離的TFPI-2完全被消化。

這一證據(jù)揭示了TFPI-2 pDNA中DNase-I的酶水解，并表明FA-PEG-PEI@SPION-CDDP在吸附TFPI-2和保護(hù)pDNA免受降解方面是可行的。因此，pDNA可以避免在到達(dá)靶細(xì)胞之前被培養(yǎng)基或血液中的DNase消化，從而在腫瘤中實(shí)現(xiàn)高效轉(zhuǎn)染。

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