文獻(xiàn)：甲胎蛋白*體修飾熒光磁性探針對(duì)HepG2細(xì)胞及*模型小鼠的 研究

鏈接：https://www.ingentaconnect.com/contentone/asp/jnn/2020/00000020/00000008/art00088

作者：陳艷帆，盧江環(huán)，楊杰，郝凱旋，李敏

節(jié)選：

為構(gòu)建一種雙靶向（磁靶向和*體靶向）多功能磁性熒光脂質(zhì)體探針，該探針具有良好的生物相容性，對(duì)早期肝*的診斷具有較高的特異性。稱取1，2-二硬脂酰-sn-甘油-3-磷酸-乙醇胺-N- [氨基（聚乙二醇）]（DSPE-PEG2000-NH2）與Cy7 N-羥基琥珀酰亞胺酯（Cy7-NHS）進(jìn)行反應(yīng)，生成DSPE-PEG 2000 -Cy7。將蛋黃卵磷脂、膽固醇、DSPE-PEG 2000 -Cy7和Fe 3 O 4依次加入到反應(yīng)液中，合成熒光四氧化三鐵（Fe 3 O 4）脂質(zhì)體。將磁性脂質(zhì)體（MLP）與甲胎蛋白（AFP）多克隆*體偶聯(lián)，并對(duì)末端AFP*體修飾的磁性脂質(zhì)體在人肝*細(xì)胞HepG2和肝*模型小鼠中的應(yīng)用進(jìn)行了分析研究。熒光四氧化三鐵脂質(zhì)體呈球形，平均流體動(dòng)力學(xué)直徑約150nm，帶負(fù)電荷。該熒光磁性探針對(duì)HepG2細(xì)胞毒性較小，并提高了AFP*體靶向成像在肝*模型小鼠肝*診斷中的特異性。總之，AFP*體偶聯(lián)的熒光磁性探針是一種很有前途的多功能工具，具有良好的生物相容性，并具有較高的靈敏度和特異性，可用于臨床*癥的診斷和*。

To construct a dual-targeting (magnetic targeting and antibody targeting) multi-functional magnetic fluorescent liposome probe with a good biocompatibility and high specificity for early liver cancerdiagnosis. 1,2-distearoyl-sn-glycero-3-phospho-ethanolamine-N-[amino(polyethylene glycol)] (DSPE-PEG2000-NH2) and Cyanine7 N-hydroxysuccinimide ester (Cy7-NHS) were measured and reacted for forming DSPE-PEG2000-Cy7. Egg yolk lecithin, cholesterol, DSPE-PEG2000-Cy7 and Fe3O4 were added in orderly into the reaction solution for synthesis of fluorescent ferroferric oxide (Fe3O4) liposome. The Magnetic liposome (MLP) was coupled with Alpha-Fetoprotein (AFP) polyclonal antibody and the ending AFP antibody modified magnetic liposome was analyzed and studied on human hepatoma cell HepG2 and cancer model mouse. The fluorescent ferroferric oxide liposome appeared as a sphericalmorphology and they were about 150 nm in mean hydrodynamic diameter with negative charge. The fluorescent magnetic probe had a little toxic effect on hepG2 cells and increased the specificity liver cancer diagnosis by AFP antibody target imaging in model mouse of liver cancer. In brief, the AFP antibody conjugated fluorescent magnetic probe is a promising multi-functional tool with good biocompatibility and high sensitivity and specificity for clinical cancer diagnosis and therapy.

西安齊岳生物提供相關(guān)產(chǎn)品：

DSPE-PEG-Mal

DSPE-RB

DSPE-PEI-Dextran

LyP-1-PEG-DSPE

Angiopep-2 PEG

DSPE-PEG-CSNRDARRC（二硬脂酰基磷脂酰乙醇胺-聚乙二醇-*靶向蛋白)

DSPE-PEG-BSA

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