文獻：Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy?

作者：Baskaran Purushothaman , Jinhyeok Choi , Solji Park , Jeongmin Lee , Annie Agnes Suganya Samson , Sera Hong and Joon Myong Song

文獻鏈接：https://pubs.rsc.org/en/content/articlehtml/2018/tb/c8tb01923a

摘要：

The release pattern of DOX from the DOX@TPP–PEG–biotin self-assembled nanoparticles was evaluated by the dialysis method. In this experiment, the released sample was measured at different time points using HPLC. Fig. 2c shows the release of DOX from the self-assembled nanoparticles at 37 °C in different PBS media (pH 7.4 and pH 6.0), indicating desirable stability of the DOX@TPP–PEG–biotin SANs. After 24 h, the DOX released was only 30.0 ± 1.8% in the pH 7.4 PBS medium compared to that in the pH 6.0 PBS medium (65.4 ± 2.0%). These results indicate that under acidic conditions the DOX was released into the cellular medium. The sizes and zeta potentials in Fig. 2a and b showed no aggregation behavior at room temperature for five days at 37 °C, indicating that the self-assembled nanoparticles prepared in this study showed adequate stability.

DOX的釋放模式DOX@TPP通過透析法對PEG-生物素自組裝納米粒子進行了評估。在該實驗中，使用HPLC在不同時間點測量釋放的樣品。

顯示了在37°C下，在不同的PBS介質（pH 7.4和pH 6.0）中，DOX從自組裝納米粒子中釋放出來，表明DOX具有穩定性DOX@TPP–PEG–生物素SAN。24小時后，與pH 6.0 PBS培養基（65.4±2.0%）相比，pH 7.4 PBS培養基中釋放的DOX僅為30.0±1.8%。

這些結果表明，在酸性條件下，DOX被釋放到細胞培養基中。圖2a和b中的尺寸和ζ電位在37°C的室溫下連續五天沒有顯示出聚集行為，表明本研究中制備的自組裝納米粒子顯示出足夠的穩定性。

相關推薦：

PLGA(5K)-PEG-NHS

Y-shape-PEG-NHS

4-ArmPEG-(3Alkyne-1NHS)

4-ArmPEG-(3Biotin-1NHS)

8-ArmPEG-(2ARM-NHS,6ARM-Biotin)

Biotin-PEG-NHS

Biotin-PEG4-S-S-NHS

mPEG-SS-NHS

NHS-PEG-NHS

PDLLA(5K)-PEG-NHS

PLGA(75:25)(5K)-PEG-NHS

sulfo NHS-PEG-SS-PEG-NHS sulfo

以上文章內容來源各類期刊或文獻，如有侵權請聯系我們刪除！