文獻：AIE-Featured Redox-Sensitive Micelles for Bioimaging and Efficient Anticancer Drug Delivery

作者：by Wei Zhao 1,?,Zixue Li 1,?,Na Liang 2,*,Jiyang Liu 1,Pengfei Yan 1 andShaoping Sun

 文獻鏈接：https://www.mdpi.com/1422-0067/23/18/10801

The drug-loaded Bi(mPEG-S-S)-TPE micelles were obtained as follows. At first, the Bi(mPEG-S-S)-TPE dispersion in distilled water was prepared. Then, the PTX in the acetone solution was added. After being sonicated for 3 min (on for 3 s and off for 2 s), the mixture was dialyzed (MWCO of 3500 Da) against distilled water for 2 h and further filtrated with a 0.45 μm filter to obtain the drug-loaded micelles. The micelles could be further freeze-dried and stored as a white powder.

XRD analysis was used to analyze the crystalline characteristic of PTX in the micelles (Geigerflex, Rigaku Co., Tokyo, Japan). Data were collected from 5° to 50° with a step-scan mode. The dynamic light-scattering method was employed to determine the particle size and zeta potential of PTX-loaded micelles (Zetasizer Nano-ZS90, Malvern Instruments, Malvern, UK). The drug loading (DL) and encapsulation efficiency (EE) were calculated using Equations (2) and (3), respectively.

Bi（mPEG-S-S）-TPE膠束的制備與表征

載藥的Bi（mPEG-S-S）-TPE膠束如下獲得。首先，制備了Bi（mPEG-S-S）-TPE在蒸餾水中的分散體。然后，加入丙酮溶液中的PTX。經過3分鐘的超聲處理（開啟3秒，關閉2秒）后，將混合物用蒸餾水透析（MWCO為3500 Da）2小時，然后用0.45μm過濾器進一步過濾，得到載藥膠束。膠束可以進一步冷凍干燥，并以白色粉末的形式儲存。

XRD分析用于分析膠束中PTX的結晶特性。采用階躍掃描模式從5°到50°收集數據。采用動態光散射法測定PTX負載膠束的粒徑和ζ電位（Zetasizer Nano-ZS90，Malvern Instruments，英國Malvern）。分別使用方程式（2）和（3）計算藥物負載量（DL）和包封效率（EE）。

相關推薦：

Silane-PEG-Biotin

LA-PEG-Biotin

IA-PEG-Biotin

Biotin-PEG-ACA

N3-PEG-Biotin

OPSS-PEG-Biotin

Biotin-PEG-Mal

Biotin-PEG-SCM

SH-PEG-Biotin

Biotin-PEG-OH

Biotin-PEG-NH2

以上文章內容來源各類期刊或文獻，如有侵權請聯系我們刪除！