文獻：同時阻斷 CD47 和 PD-L1 可增強先天性和適應性癌癥免疫反應以及細胞因子釋放

鏈接：https://www.thelancet.com/article/S2352-3964(19)30158-6/fulltext

作者：舒 蓮，謝銳 志，葉玉英，謝曉東，李淑慧，路玉生，李碧飛，程云龍，弗拉基米爾·卡塔納耶夫，李嘉

節選：

MAL-PEG-DOPE的合成

MAL-PEG-DOPE的合成參考以前發表的文章。采用EDC/NHS技術將MAL-PEG-COOH的羧基與DOPE的胺基結合。具體方法如下：將30mg羧基修飾的PEG溶于二氯甲烷中，并與5mgEDC和4mgNHS混合，室溫下連續攪拌2h。然后加入8mg DOPE（MAL-PEG-COOH∶DOPE=1∶1，摩爾比），氮氣保護下反應過夜。將反應產物在旋轉蒸發儀中干燥至大部分二氯甲烷，然后加入冷乙腈中。未反應的DOPE在2414g下離心10min，不溶于冷乙腈。上清液在旋轉蒸發儀中干燥為稀脂質。將膜用DD水水化。將反應產物裝入透析袋（分子量= 8 k Da）中，并轉移至50 mL DD水溶液中，室溫下反應48小時，分離游離的EDC/NHS/MAL-PEG-COOH。最終產物DOPE-PEG-MAL隨后用凍干機冷凍。為了驗證DOPE-PEG-MAL的結合，對樣品進行了核磁共振波譜分析。

Synthesis of MAL-PEG-DOPE

Synthesis of MAL-PEG-DOPE refers to previously published articles [22,23]. The conjugation of carboxyl groups of MAL-PEG-COOH to the amine groups of DOPE was accomplished using the EDC/NHS technique. The process was carried out as follows: 30?mg carboxyl-modified PEG was dissolved in dichloromethane and mixed with EDC (5?mg) and NHS (4?mg). The solution was stirred continuously for 2?h at room temperature. Subsequently, 8?mg DOPE (MAL-PEG-COOH: DOPE =1:1, molar ratio) was added, and the reaction proceeded overnight under nitrogen. The reaction product was dried out most dichloromethane in rotary evaporator and then added to cold acetonitrile. The unreacted DOPE was centrifuged at 2414g for 10?min which was insoluble in cold acetonitrile. And the supernatant was dried to thin lipid in rotary evaporator. The film was hydrated with DD water. The reaction product was enclosed in dialysis bag (MW?=?8?k?Da) and transferred into 50?mL of DD water solution to separate free EDC/ NHS/ MAL-PEG-COOH at room temperature for 48?h. The final product DOPE-PEG-MAL was subsequently freezed by lyophilizer. To confirm the DOPE-PEG-MAL conjugation, the samples were examined by nuclear magnetic resonance spectroscopy.

西安齊岳生物提供相關產品：

DSPE-PEG-NBD

DSPE-PEG-PDP

DSPE-PEG-Anti-rat CC531mAb（二硬脂酰基磷脂酰乙醇胺-聚乙二醇-*靶向蛋白)

DSPE-OPSS

DSPE-PEG-SC

LyP-1-PEG-DSPE

Cy7-DSPE

TAT-PEG-DSPE

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