文獻：聚乙二醇負載脂質雙層在金表面的原位形成及表征

鏈接：https://pubs.acs.org/doi/abs/10.1021/la048378o

作者：杰弗里·C·芒羅，柯蒂斯·W·弗蘭克

摘要：

有人提出，在脂質雙層膜和固體表面之間添加聚合物墊層，可以形成柔軟、可變形的膜層，從而實現跨膜蛋白的插入和遷移。本研究通過兩步吸附過程，在聚乙二醇 (PEG) 載體上形成了可遷移的、可束縛的脂質雙層膜。 PEG 薄膜是通過共吸附異功能遠爪 PEG 脂質聚合物（1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-N-聚（乙二醇）-2000- N- [3-（2-（吡啶基二硫代）丙酸酯]）（DSPE-PEG-PDP）和非脂質功能化的 PEG-PDP 從乙醇/水混合物中制備的，如先前的論文（Munro，JC；Frank，CW Langmuir 2004，20，3339-3349）中所述。

然后使用兩步脂質吸附策略。首先，將脂質從己烷溶液中吸附到 PEG 載體上。其次，將囊泡吸附并融合在表面以在水環境中形成雙層。光漂白實驗后的熒光恢復表明，該過程產生擴散系數約為 2 μm /s。隨著束縛脂質密度的增加，雙層膜的遷移率略有降低。表面等離子體共振法（用于測定原位膜厚度）和熒光法（用于定量測定每個18 x 18毫米樣品的熒光強度）也證實了雙層膜的形成，而非多層結構。遺憾的是，熒光顯微鏡檢查也顯示樣品上存在較大的缺陷，這限制了該系統的實用性。

Abstract Image

Inclusion of a polymer cushion between a lipid bilayer membrane and a solid surface has been suggested as a means to provide a soft, deformable layer that will allow for transmembrane protein insertion and mobility. In this study, mobile, tethered lipid bilayers were formed on a poly(ethylene glycol) (PEG) support via a two-step adsorption process. The PEG films were prepared by coadsorbing a heterofunctional, telechelic PEG lipopolymer (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-poly(ethylene glycol)-2000-N-[3-(2-(pyridyldithio)propionate]) (DSPE-PEG-PDP) and a nonlipid functionalized PEG-PDP from an ethanol/water mixture, as described in a previous paper (Munro, J. C.; Frank, C. W. Langmuir2004, 20, 3339?3349). Then a two-step lipid adsorption strategy was used. First, lipids were adsorbed onto the PEG support from a hexane solution. Second, vesicles were adsorbed and fused on the surface to create a bilayer in an aqueous environment. Fluorescence recovery after photobleaching experiments show that this process results in mobile bilayers with diffusion coefficients on the order of 2 μm2/s. The mobility of the bilayers is decreased slightly by increasing the density of tethered lipids. The formation of bilayers, and not multilayer structures, is also confirmed by surface plasmon resonance, which was used to determine in situ film thickness, and by fluorimetry, which was used to determine quantitatively the fluorescence intensity for each 18 by 18 mm sample. Unfortunately, fluorescence microscopy also shows that there are large defects on the samples, which limits the utility of this system.

西安齊岳生物提供相關產品：

DSPE-PEG-Galactose

DSPE-PEG-Dextran

DSPE-PEG-Chitosan

DSPE-PEG-Mannose

DSPE-PEG-Glucose

DSPE-PEG-HA

DSPE-PEG-Alginate

DSPE-PEG-Ficoll

DSPE-PEG-Inulin

DSPE-PEG-lactosyl

DSPE-PEG-Chitin

DSPE-PEG-Rhamnose

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