文獻：PLGA-卵磷脂-PEG核殼納米粒子用于癌癥靶向*

鏈接：https://www.worldscientific.com/doi/abs/10.1142/S1793984411000359

作者：艷麗， 里克·奧克塔維安蒂·托伊普， 陶鵬， 和 林詩琳

摘要：

我們報道了一種多功能聚乳酸-乙醇酸共聚物 (PLGA)-卵磷脂-聚乙二醇 (PEG) 核殼納米粒子 (NPs)，該納米粒子兼具脂質體和聚合物納米粒子的優點，可用于遞送化療藥物。該納米粒子的粒徑、表面電荷和表面官能團可通過各種配方參數輕松調節，且重復性高，例如脂質/聚合物、1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺 (DSPE)-PEG- COOH /卵磷脂、DSPE-PEG- COOH /DSPE-PEG- NH 2 的質量比以及 DSPE-PEG 端基的修飾。我們將模型化療藥物——親水性順鉑 (DDP) 或疏水性 DDP 前藥——封裝于納米顆粒 (NP) 中，結果顯示其包封率高、穩定性佳、對高 FA 受體表達的 MCF-7 細胞具有特異性靶向識別能力，且 FA 受體表達量高，且細胞毒性較小。此類 PLGA-卵磷脂-PEG 核殼納米顆粒 (NP) 已被證明是一種*具潛力的癌癥靶向*藥物遞送納米載體。

Abstract：

Protein cages have been widely investigated as molecular drug carrier. E2 protein from Bacillus stearothermophillus forms a dodecahedral cage structure of approximately 24 nm in diameter. To formulate a sustainable release profile, E2 protein was further encapsulated into poly(lactide-co-glycolide) (PLGA) microparticles to form a composite structure using water-in-oil-in-water (W/O/W) double emulsion method. The influence of fabrication parameters on microparticle morphology and E2 protein release profile were investigated. The microparticle size increased when the stirring speed of the second emulsification decreased. Decrease in the volume of external aqueous phase led to the reduction of microparticle size without affecting its porosity. The higher ionic concentration of external aqueous phase in the presence of surfactant resulted in microparticles with closed pores on surface. Increase in polymer concentration also led to the formation of less porous microparticles. The E2 protein was not dissociated upon encapsulation into PLGA microparticles based on the unchanged particle size of E2 protein. E2 protein release was studied in phosphate-buffered saline solution at 37°C. The initial burst and release rate were lowered as the surfactant concentration in external water phase during the fabrication process was increased from 0.1% to 1% (w/v). After 14-day incubation, no observable polymer degradation was found while the surface of microparticles appeared to be smoother than before incubation.

西安齊岳生物提供相關產品：

VIP-PEG-DSPE

CREKA-PEG-DSPE

Asp8-PEG-DSPE

WYRGRL-PEG-DSPE

CTP-PEG-DSPE

CMP-PEG-DSPE

SHp-PEG-DSPE

CGKRK-PEG-DSPE

Xpeptide-PEG-DSPE

Asp6-PEG-DSPE

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