鏈接:https://www.sciencedirect.com/science/article/pii/S2405580821001618
作者:濱進(jìn) 酒井美嘉 板倉翔子 真島英二 小暮健太郎
節(jié)選:
NIH3T3細(xì)胞(小鼠成纖維細(xì)胞株)購自日本理化學(xué)研究所BRC細(xì)胞庫(日本和光)。A375細(xì)胞(人黑色素瘤細(xì)胞株)和HUEhT-2細(xì)胞(永生化人臍靜脈內(nèi)皮細(xì)胞株)購自日本JCRB細(xì)胞庫(日本大阪)。3-(N-琥珀酰亞胺基氧戊二酰)氨基丙基聚乙二醇-氨基甲酰二硬脂酰磷脂酰乙醇胺(DSPE-PEG-NHS)、N-(琥珀酰亞胺基氧戊二酰)-L-α-磷脂酰乙醇胺(二硬脂酰)(DSPE-NHS)和1,2-二硬脂酰-sn-甘油-3-磷酸膽堿(DSPC)購自日本油脂株式會社(日本東京)。膽固醇、磷酸雙十六烷基酯(DCP) 和DXR 鹽酸鹽購自 Sigma-Aldrich 公司(美國密蘇里州圣路易斯)。1,2-二油酰-sn-甘油-3-磷酸乙醇胺-N-(麗絲胺羅丹明B磺酰基) (Rh-PE) 購自 Avanti Polar Lipid 公司(美國阿拉巴馬州阿拉巴斯特)。*人 CD31 *體(小鼠IgG1 κ)、*人 CD147 *體(小鼠 IgG1 κ)和小鼠 IgG1 κ 同型對照購自 eBioscience 公司(美國加利福尼亞州圣地亞哥)。四甲基羅丹明(TRITC) 標(biāo)記的兔*鼠多克隆*體購自 Dako 公司(丹麥格洛斯特魯普)。
譯文:
NIH3T3 cells, a mouse fibroblast cell line, were obtained from RIKEN BRC Cell Bank (Wako, Japan). A375 cells, a human melanoma cell line, and HUEhT-2 cells, an immortalized human umbilical vein endothelial cell line were obtained from the JCRB Cell Bank (Osaka, Japan). 3-(N-succinimidyloxyglutaryl) aminopropyl, polyethyleneglycol-carbamyl distearoylphosphatidyl-ethanolamine (DSPE-PEG-NHS), N-(Succinimidyloxy-glutaryl)-L-α-phosphatidylethanolamine, Distearoyl (DSPE-NHS) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) were obtained from NOF Corporation (Tokyo, Japan). Cholesterol, dihexadecyl phosphate (DCP) and DXR hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rh-PE) were purchased from Avanti Polar Lipid (Alabaster, AL, U.S.A.). Anti-human CD31 antibody (mouse IgG1 κ), anti-human CD147 antibody (mouse IgG1 κ) and mouse IgG1 κ isotype control were purchased from eBioscience (San Diego, CA, USA). Tetramethylrhodamine (TRITC)-labeled rabbit anti-mouse polyclonal antibody was obtained from Dako (Glostrup, Denmark).
西安齊岳生物提供相關(guān)產(chǎn)品:
DSPE-PEG-iRGD
DSPE-PEG-多肽(各種定制)
DSPE-PEG-RGD
DSPE-PEG-c(RGDfk)
DSPE-PEG-c(RGDfK)-FITC
DSPE-PEG-c(RGDfK)-Biotin
DSPE-PEG-c(RGDfc)
DSPE-PEG-GE11
DSPE-PEG-PTP
DSPE-PEG-T7(HAIYPRH)
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