鏈接：https://www.sciencedirect.com/science/article/pii/S1549963420301921

作者：趙 章亭，馬興 元，張瑞 環，胡發彪，張 桐，劉玉萍，Myong Hun Han，方 有，楊 易，鄭文云

摘要：

DNA疫苗是預防和*傳染病的一個有吸引力的免疫平臺，但現有的缺點限制了它在臨床前和臨床試驗中的應用，例如免疫原性弱和半衰期短。本文，我們報道了一種新型脂質體-聚合物混合納米顆粒（pSFV-MEG/LNPs），由可生物降解的核心（mPEG-PLGA）和親水外殼（卵磷脂/PEG-DSPE-Mal 2000）組成，用于遞送多表位自復制DNA疫苗（pSFV-MEG）。與PBS相比，具有最佳粒徑（161.61±15.63nm）和高包封率（87.60±8.73％）的pSFV-MEG/LNPs可誘導強烈的體液免疫反應（3.22倍）和細胞免疫反應（1.60倍）。此外，pSFV-MEG/LNPs的體液和細胞免疫應答分別是pSFV-MEG的1.58倍和1.05倍。所有結果證實，LNPs是一種非常有前景的增強pSFV-MEG體液和細胞免疫應答的工具。此外，該合理的設計和遞送平臺可用于開發其他傳染病的DNA疫苗。

譯文：

DNA vaccine is an attractive immune platform for the prevention and treatment of infectious diseases, but existing disadvantages limit its use in preclinical and clinical assays, such as weak immunogenicity and short half-life. Here, we reported a novel liposome-polymer hybrid nanoparticles (pSFV-MEG/LNPs) consisting of a biodegradable core (mPEG-PLGA) and a hydrophilic shell (lecithin/PEG-DSPE-Mal 2000) for delivering a multi-epitope self-replication DNA vaccine (pSFV-MEG). The pSFV-MEG/LNPs with optimal particle size (161.61?±?15.63 nm) and high encapsulation efficiency (87.60?±?8.73%) induced a strong humoral (3.22-fold) and cellular immune responses (1.60-fold) compared to PBS. Besides, the humoral and cellular immune responses of pSFV-MEG/LNPs were 1.58- and 1.05-fold than that of pSFV-MEG. All results confirmed that LNPs was a very promising tool to enhance the humoral and cellular immune responses of pSFV-MEG. In addition, the rational design and delivery platform can be used for the development of DNA vaccines for other infectious diseases.

西安齊岳生物提供相關產品：

DSPE-PEG-CPP

DSPE-PEG-octreotide

DSPE-PEG-SP94

DSPE-PEG-CCK8

DSPE-PEG2000-GE11

DSPE-PEG2K-YIGSR

DSPE-PEG2K-RVG29

DSPE-PEG2K-MMPs

DSPE-PEG2000-NGR

DSPE-PEG-GRGDS

DSPE-PEG2K-R8

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