DSPE-PEG-PEI介導的聯合遞藥系統在肝癌中的應用研究

鏈接：https://xueshu.baidu.com/usercenter/paper/show?paperid=13620650816s00y0k60q02j0k0742988&site=xueshu_se

作者： G Tian，R Pan，B Zhang，M Qu，J Wu

摘要：Combination therapy based on nano-sized drug delivery system has been developed as a promising strategy by combining two or more anti-tumor mechanisms. Here, we prepared liver-targeted nanoparticles (GH-DPP) composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethylene glycol-polyetherimide (DSPE-PEG-PEI) with Glycyrrhetinic acid-modified hyaluronic acid (GA-HA) for co-delivery of doxorubicin (DOX) and Bcl-2 siRNA. Particles size, zeta potential and morphology were determined for the drug-loaded GH-DPP nanoparticles (siRNA/DOX/GH-DPP). Cellular uptake and in vitro cytotoxicity were analyzed against HepG2 cells. In vivo bio-distribution and anti-tumor therapeutic effects of siRNA/DOX/GH-DPP were evaluated in H22-bearing mice. The results showed that siRNA/DOX/GH-DPP nanoparticles were nearly spherical and showed dose-dependent cytotoxicity against HepG2 cells. Compared to Glycyrrhetinic acid-free co-delivery system (siRNA/DOX/DPP) and GH-DPP nanoparticles for delivery of DOX or Bcl-2 siRNA alone, siRNA/DOX/GH-DPP nanoparticles could induce more cellular apoptosis, and showed higher anti-tumor effect. Herein GH-DPP nanoparticles could simultaneously deliver both chemotherapy drugs and siRNA into the tumor region, exhibiting great potential in anti-tumor therapy.

譯文：

通過結合兩種或多種**機制，已將基于納米級藥物遞送系統的聯合療法發展為一種有前途的策略。在這里，我們制備了肝靶向納米顆粒（GH-DPP），其由1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-聚乙二醇-聚醚酰亞胺（DSPE-PEG-PEI）與甘草次酸修飾的透明質酸（GA- HA）用于共同遞送阿霉素（DOX）和Bcl-2 siRNA。確定了載藥的GH-DPP納米顆粒（siRNA / DOX / GH-DPP）的粒徑，ζ電位和形態。分析了針對HepG2細胞的細胞攝取和體外細胞毒性。在荷H22小鼠中評估了siRNA / DOX / GH-DPP的體內生物分布和***作用。結果表明，siRNA / DOX / GH-DPP納米顆粒接近球形，并且對HepG2細胞顯示出劑量依賴性的細胞毒性。與單獨使用DOG或Bcl-2 siRNA的無甘草次酸共遞送系統（siRNA / DOX / DPP）和GH-DPP納米顆粒相比，siRNA / DOX / GH-DPP納米顆粒可以誘導更多的細胞凋亡，并且顯示出更高的凋亡率。**作用。此處，GH-DPP納米顆粒可同時將化療藥物和siRNA送入*區域，在***中顯示出巨大的潛力。

DOI：10.3389/fphar.2019.00004

西安齊岳生物提供相關產品：

DSPE-RB

DSPE-FITC

DSPE-ICG

DSPE-OPSS

DSPE-PCL

DSPE-PEI

DSPE-PEI-Dextran

DSPE-PLA

DSPE-PLGA

DSPE-Poly(L-glutamic acid)

DSPE-Poly(L-lysine)

DSPE-Pyrene

DSPE-Cap-Biotin

DSPE-Cap-Folate

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